Friday, August 15, 2014

What occurs after pollination that leads to fertilization of the egg cell in a flower?The pollen of the flower is carried from the anther of the...

An experiment you could perform could test the hypothesis
that pollen germinates in the pistil and makes a tube through which the sperm and egg
can unite in the ovary, leading to fertilization, due to a chemical signal from the
ovary. While you cannot see inside the stigma and style, you can do an experiment that
shows germination of a pollen grain on a slide. Pollen grains can be transferred to a
slide by tapping an anther, the male reproductive part of a plant,to release pollen.
Next, you can add either a nutrient solution with calcium or crushed ovary to the slide
and watch to see what direction a pollen tube will grow. That set-up would be the
experimental set-up. As a control, you can add pollen to a different slide and a
solution containing just water. Again, observe the growth(if any) of a pollen tube. If
your hypothesis is correct, the direction of pollen tube germination should be toward
calcium (which is a chemical signal) or toward bits of the ovary. Good
luck.

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